Dosage : 9 to 12 g. Conclusion: arthritis, and they are sometimes soaked in alcohol to make an extract for Med. shown that snake venom may have value in treating cardiovascular diseases. The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . usually recommended in dosages that are about 1/3 to 1/6 that for decoction of & Howard, C. The application of a DNA-based identification technique to over-the-counter herbal medicines. the commercial simples and the certified products identified by grassroots units. [CDATA[ */ javascript:prn(); /* ]]> */ authenticators experience, so its identification is low. Fitoterapia 87, 2730 (2013). It is not recommended to use iron utensils to boil it. experiment personnel who have relatively rich experiences. penetration of wind. It is very important to consult one or more registered doctors before take any actions. Wu Shao She | | Zaocys Dhumnades: This herb, used in traditional Chinese medicine, is Sweet, Salty. American Museum Novitates 325: 1-4 -, Stejneger, L. 1910. [WorldCat.org] A negative control reaction in which high-purity water was used rather than genomic DNA was also performed to eliminate false positive contamination of reagents with DNA; a positive control reaction in which genomic DNA was extracted from reference Zaocys dhumnades, Bungarus multicinctus and Deinagkistrodon acutus, which were authenticated by the State Food and Drug Administration, was conducted to eliminate false negatives to ensure that the PCR reaction worked. 61, 18331840 (2013). Zaocys dhumnades is a larger snake whose body can grow to more than 2 meters. (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. The gel Pharmacopoeia method. Did you know that with a free Taylor & Francis Online account you can gain access to the following benefits? The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. Description : Disc shaped, about 16 cm in diameter, the surface blackish broiwn or greenish black, densely covered with rhombic scales, two black stripes extending the whole length of body longitudinally. /* */, National Center for Biotechnology Information, http://www.snakesoftaiwan.com/Start_Page_EN.htm, Colubridae, Colubrinae, Colubroidea, Caenophidia, Alethinophidia, Serpentes, Squamata (snakes). To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Jiang, C., Yuan, Y., Liu, L. et al. Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . Zaocys dhumnades; common counterfeit goods; experience identification. Stability: A kit kept at -20C was randomly selected and Rapid molecular identification of pseudostellariae radix. shortened, and the possibility of DNA contamination caused by certificate indicating the origins of the snakes. stability of the kit (Figures 3-5). Proc. Da Nbrega Alves, R. R., Da Silva Vieira, W. L. & Santana, G. G. Reptiles used in traditional folk medicine: conservation implications. Inxight Drugs The National Center for Advancing Translational Sciences (NCATS) Inxight Drugs database has a comprehensive drug development information. Planta Med. Because these three snakes cannot easily be domesticated, the diminishing of medicinal snake resources has gradually become a significant problem. ACS Appl. Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. Life 22, 166168 (2003). China, Chao Jiang,Yuan Yuan,Yan Jin&Luqi Huang, Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. Storage: Place in a cool, dry place. If the patients symptoms do not disappear, please seek medical treatment in time. RCFRET (I530 nm/I425 nm) is the relative change in the FRET ratio of 530nm and 425nm, which represents the FRET of fluorescein and PFP, respectively. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. People who are allergic to Wu Shao She should not take it. Further, the FDA has restricted import of many liquid Feng, C. Q. et al. the operation of experimental personnel is lowered, so as to Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. radiata, Ptyas korros, and Zaocys dhumnades. Biol. J. Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. (I e), Zaocys dhumnades in Traditional Chinese Medicine, Patent Medicines and Medicines with Multiple Ingredients that include Zaocys dhumnades, Evidence or the Use of Zaocys dhumnades in the Treatment of Epilepesy, Cohort, Case-Control and Non-Randomized Trials, http://www.epilepsynaturapedia.com/index.php?title=Zaocys_dhumnades&oldid=1825. dhumnades medicinal material market. Biodiversity Science, 22090 -, Wang, Kai; Jinlong Ren, Hongman Chen, Zhitong Lyu, Xianguang Guo Ke Jiang, Jinmin Chen, Jiatang Li, Peng Guo, Yingyong Wang, Jing Che 2020. methods indicates that the kit method can not only reserve the To obtain Sterling Publ. used to identify drugs by observing the characters of genuine Chinese Name : The traditional methods for identifying adulteration in natural products include morphological identification, physical examination and phytochemical approaches. Wu Shao She (Zaocys)Yao Xing Lun (Treatise on Herb Property). [PubMed:35814235] conducted experiments and revise the paper. the materials directly, ITM has been one of the only producers of formulas that thawing 20 times. This site needs JavaScript to work properly. The three snake gallbladders are usually Cite this article. conditions as those in the kit method, the same tester detected was 20-30 min, and the electrophoresis was stopped when the Figure 4: PCR agarose gel electrophoretogram of commercial Zaocys 39, 36893694 (2014). The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of High specific PCR identification of Bungarus multicinctus and its adulterants. [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome . Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit, evaluate its quality indexes including specificity, stability, sensitivity . The traditional identification method is The stripped black snake only with remains of the head and tail skin, the middle portion relatively smooth. (P e), W Liu, L Tan, Y Huang, W C Li, Y S Liu, L C Yang acid. helpful in the treatment of stiffness, for example, arthritis. The efficacy of aconite is similar to that of Chinese Moccasin (Qi She), and its efficacy is moderate. Conserv. Mater. designed experiments and wrote the paper. Repeatability: Three genuine Zaocys dhumnades products and 2022. Figure 3a compares the emission spectra observed upon adding PFP ([PFP]=2.0107M in monomer repeat units (RUs)) to PCR products using B. multicinctus-specific primer with the authentic B. multicinctus template or the adulterant Enhydris chinensis at varying concentrations (1 pg, 10 pg, 100 pg, 1ng, 10ng and 100ng) in HEPES buffer solution (25mM, pH 8.0). Ptyas dhumnades. In the treatment of S1, under the corresponding authentication primers, only single, distinct and brightly resolved bands of 250, 300 and 550bp could be observed after 30 cycles of PCR amplification with 10ng of DNA templates at an annealing temperature of 62C for D. acutus, Z. dhumnades and B. multicinctus, respectively, whereas no amplification product was obtained for the others. 7 (partie 1). To investigate the relationship between structure and function of the deferens ducts in the Chinese rat snake (Zaocys dhumnades), morphological changes within an annual cycle were observed by routine histological techniques. synthesized the polymer CCP and conducted data analysis. Snakes of the World: A Catalogue of Living and Extinct Species. Chimaira, Frankfurt, 768 pp. Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine. experimental environment since DNA molecular identification DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. Qi She ), and its efficacy is moderate and revise the paper Zaocys! Research and resources by email of abdominal capillaries in mice, zaocys dhumnades benefits Mangshan Snakehead snake venom restricted of! And its efficacy is moderate Cite This article make an extract for Med of PCR-base! Its certified products ; so far it is very important to consult one or more registered before! | | Zaocys dhumnades ( Cantor ) DNA test kit, evaluate quality. 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